BIOL 347L. Introduction to Cell and Stem Cell Culture Lab (3)

Prerequisites: BIOL 106, BIOL 106L, BIOL 107 and BIOL 107L. Recommended Preparatory: BIOL 380. Introduction to theory and techniques used in cell and stem cell culture research. Students learn through hands-on experience in the laboratory the experimental design, technical mechanisms, and interpretation of commonly used techniques in cell culture including: issues of laboratory safety, aseptic technique, media choice and preparation, cell cryoretrieval, cell viability, cell dilution, and passaging, cell counts and/or confluency evaluation, growth curves, cryopreservation of adherent and suspension cell lines, and culture of several stem cell lines using feeder-free technology. Lab 9 hours.